GDF-8 (Myostatin) Recombinant Protein 1mg/Vial | Research Grade

CAS No.: 271597-12-7
Brand: ACDC
Purity: ≥99% (SDS-PAGE verified)

GDF-8, also known as Myostatin, is a member of the transforming growth factor-beta (TGF-β) superfamily and plays a critical role in regulating skeletal muscle growth. The protein is encoded by the MSTN gene and acts as a natural inhibitor of myogenesis.

Recombinant GDF-8 protein is widely used in biomedical research related to muscle atrophy, metabolic disorders, and muscle growth regulation. By activating the Smad2/3 signaling pathway, Myostatin suppresses myoblast proliferation and differentiation, making it an important tool for studying muscle physiology and drug discovery targeting muscle-wasting diseases.

This research-grade GDF-8 recombinant protein is produced in CHO expression systems and supplied as sterile lyophilized powder suitable for laboratory applications.

What is GDF-8 (Myostatin)?

Myostatin (Growth Differentiation Factor-8) is a secreted signaling protein that negatively regulates skeletal muscle development. It was first identified as a key regulator controlling muscle mass in mammals.

Mutations in the MSTN gene have been shown to cause significant muscle hypertrophy in animals and humans, which highlights the importance of this protein in muscle homeostasis.

Because of its role in muscle growth regulation, GDF-8 has become a major target in therapeutic research for muscle wasting diseases, including:

• Muscular dystrophy

• Sarcopenia

• Cachexia

• Metabolic syndrome

Product Specifications

Product Name
GDF-8 (Myostatin) Recombinant Protein

Expression System
CHO cells

Molecular Weight
Precursor protein ~25 kDa
Active dimer ~12.5 kDa

Amino Acid Sequence
Asn 24 – Ser 375

Purity
≥99% by SDS-PAGE and SEC-MALS

Endotoxin Level
≤0 EU/μg (LAL / rFC method)

Appearance
Sterile white lyophilized powder

Packaging
1 mg per vial
10 vials per box

Research Applications

GDF-8 recombinant protein is widely used in biomedical and pharmaceutical research.

Muscle Growth Regulation Studies

Myostatin inhibits muscle cell differentiation and proliferation by activating the Smad signaling pathway, making it a key model for studying muscle development.

Muscle Atrophy Disease Models

Researchers use GDF-8 to simulate muscle wasting conditions, enabling the study of diseases such as muscular dystrophy and cachexia.

Drug Discovery and Screening

GDF-8 provides a useful screening platform for compounds targeting muscle-wasting disorders, especially inhibitors of the TGF-β signaling pathway.

Cell Culture Experiments

The protein is commonly applied in C2C12 myoblast models to evaluate the effects of signaling molecules on myogenesis.

Recommended Usage

In Vitro Experiments

Typical concentration:
10–100 ng/mL in cell culture medium

Treatment duration:
24–72 hours depending on experimental design.

In Vivo Research

Suggested dosage range:
100–500 μg/kg body weight

Administration methods may include:

• Intraperitoneal injection

• Subcutaneous injection

Treatment period typically ranges from 7–14 days in experimental animal models.

Storage and Stability

Lyophilized Powder

Long-term storage:
-80°C (up to 36 months)

Short-term storage:
-20°C (up to 12 months)

Reconstituted Solution

Reconstitute with sterile PBS (pH 7.4) according to the Certificate of Analysis (COA).

Once reconstituted:

• Store at 2–8°C

• Use within 24 hours

• Avoid repeated freeze-thaw cycles

Shipping Information

Place of Origin: China
Shipping Port: Guangzhou

Products are shipped with:

• Dry ice temperature control

• Shock-resistant packaging

• Discreet labeling for international shipping

Typical delivery time: 48 hours after dispatch depending on destination.

Important Notice

This product is intended for research use only (RUO).
It is not approved for human consumption or clinical use.

All experiments should be conducted by qualified professionals in controlled laboratory environments.